Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Par un écrivain mystérieux
Description
Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.mdpi.com/polymers/polymers-15-01763/article_deploy/html/images/polymers-15-01763-g001.png)
Polymers, Free Full-Text
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High-resolution structure determination of sub-100 kDa complexes
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://app.jove.com/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F64061.jpg&w=3840&q=75)
JoVE Peer Reviewed Scientific Video Journal - Methods and Protocols
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.jove.com/files/ftp_upload/64061/64061fig06large.jpg)
Preparation of Nucleosome Core Particles Complexed with DNA Repair
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://ars.els-cdn.com/content/image/1-s2.0-S2211124723011464-fx1.jpg)
Cryo-ET study from in vitro to in vivo revealed a general folding
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.jove.com/files/ftp_upload/64061/64061fig02large.jpg)
Preparation of Nucleosome Core Particles Complexed with DNA Repair
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.jove.com/files/ftp_upload/64061/64061fig05large.jpg)
Preparation of Nucleosome Core Particles Complexed with DNA Repair
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.science.org/cms/10.1126/sciadv.adf7586/asset/3d037c63-dc2f-4b4a-a644-4668186683c0/assets/images/large/sciadv.adf7586-f1.jpg)
Cryo-EM structure of the human Sirtuin 6–nucleosome complex
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.pnas.org/cms/10.1073/pnas.2206542119/asset/b5f829bf-dbad-4ad7-b375-43f03bac0be1/assets/images/large/pnas.2206542119fig05.jpg)
Cryo–electron microscopy structure of the H3-H4 octasome: A
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.science.org/cms/asset/4ac36828-8647-42ea-815d-36dfcc7c7618/keyimage.gif)
Structural basis of telomeric nucleosome recognition by shelterin
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://royalsocietypublishing.org/cms/asset/26501e2d-a72e-48ae-8e87-2b9e215d4e6a/rsob170255f03.jpg)
Cryo-EM structure of the nucleosome containing the ALB1 enhancer
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://www.biorxiv.org/content/biorxiv/early/2021/10/28/2021.10.27.466091/F4.medium.gif)
Cryo-electron microscopy structure of the H3-H4 octasome without
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Structural basis for binding diversity of acetyltransferase p300
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